Beta-TC-6 小鼠胰島素瘤胰島β細(xì)胞
ATCC® Number: CRL-11506?
Designations: Beta-TC-6
Depositors: CytoTherapeutics, Inc.
Biosafety Level: 2 [Cells contain SV40 viral DNA Sequences ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus, transgenic for SV40 large T antigen (mouse, transgenic for SV40 large T antigen)
Morphology: epithelial
CRL-11506 Beta-TC-6 小鼠胰島素瘤胰島β細(xì)胞
Source: Organ: pancreas
Disease: insulinoma
Cell Type: beta cell;
Cellular Products: insulin, glucagon and somatostatin
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: The cell line was derived from a pancreatic tumor (insulinoma) arising in a transgenic mouse.
The mouse carried the pseudogene construct composed of the SV40 early region controlled by the rat insulin II gene promotor.
The cells contain abundant insulin and small amounts of glucagon and somatostatin. They secrete insulin in response to glucose. [46884]
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 15%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
CRL-11506 Beta-TC-6 小鼠胰島素瘤胰島β細(xì)胞Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
References: 46126: Laurance ME, et al. Glucose responsive insulin secreting beta-cell lines and method for producing same. US Patent 5,773,255 dated Jun 30 1998
46884: Poitout V, et al. Morphological and functional characterization of beta TC-6 cells--an insulin-secreting cell line derived from transgenic mice. Diabetes 44: 306-313, 1995. PubMed: 7533732
46885: Poitout V, et al. Insulin-secreting cell lines: classification, characteristics and potential applications. Diabetes Metab. 22: 7-14, 1996. PubMed: 8697299
CRL-11506 Beta-TC-6 小鼠胰島素瘤胰島β細(xì)胞
