產(chǎn)品[
CHOK1倉(cāng)鼠卵巢細(xì)胞亞株
]資料
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產(chǎn)品名稱:
CHOK1倉(cāng)鼠卵巢細(xì)胞亞株
產(chǎn)品型號(hào):
CRL-9618
產(chǎn)品廠商:
美國(guó)標(biāo)準(zhǔn)生物品收藏中心（ATCC）
產(chǎn)品文檔:
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簡(jiǎn)單介紹
CRL-9618 CHO-K1 倉(cāng)鼠卵巢細(xì)胞亞株，原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種；細(xì)胞庫(kù)管理規(guī)范，提供的細(xì)胞株背景清楚，提供參考文獻(xiàn)和培養(yǎng)條件！
CHOK1倉(cāng)鼠卵巢細(xì)胞亞株
的詳細(xì)介紹
CRL-9618 CHO-K1 倉(cāng)鼠卵巢細(xì)胞亞株
ATCC® Number: CRL-9618?
Designations: CHO-K1
Depositors: K Thomae
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Cricetulus griseus (hamster, Chinese)
Morphology: epithelial
Source: Organ: ovary
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: transfection host (Roche FuGENE® Transfection Reagents
Nucleofection technology from Lonza)
Gender: female
Comments: This is the same line as ATCC CCL-61.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Once or twice between subculture
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004
recommended serum:ATCC 30-2020
