CRL-1582 MOLT-4 人急性**母細(xì)胞白血病細(xì)胞
ATCC® Number: CRL-1582?
Designations: MOLT-4
Depositors: J Minowada
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens (human)
Morphology: lymphoblast
Source: Disease: acute lymphoblastic leukemia
Cell Type: T lymphoblast;
Cellular Products: high levels of terminal deoxynucleotidyl transferase (TdT) are produced [22735]
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Applications: transfection host (Roche FuGENE® Transfection Reagents
Nucleofection technology from Lonza)
Tumorigenic: Yes
Antigen Expression: CD1 (49%), CD2 (35%), CD3 A (26%) B (33%) C (34%), CD4 (55%), CD5 (72%), CD6 (22%), CD7 (77%)
Cytogenetic Analysis: This is a human cell line with the hypertetraploid chromosome number. The modal chromosome number was 95 occurring in 24% of cells. The rate of cells with higher ploidies was 0.8%. The 6q- and t(7;7) were paired and also common to all cells. The i(17q) was single and occurred only in some cells. Most normal chromosomes had four copies per cell except for the paired N7, X and Y, and two to three copies for N17.
Age: 19 years
Gender: male
Comments: The line was established from cells taken from a patient in relapse. [22524]
The patient had received prior multidrug chemotherapy. [22524]
There is a G -> A mutation at codon 248 of the p53 gene. [23322]
P53 is not expressed. [23322]
The cells do not produce immunoglobulin or Epstein-Barr virus. [22524]
MOLT-4 was derived from the same patient as the MOLT-3 cell line (ATCC CRL-1552). [22524]
The line was originally contaminated with mycoplasma but has been cured with antibiotics.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start new cultures at 4 X 10(5) cells/ml and subculture before the cell density reaches 2 X 10(6) cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
derived from same individual:ATCC CRL-1552
References: 22524: Minowada J, et al. Rosette-forming human lymphoid cell lines. I. Establishment and evidence for origin of thymus-derived lymphocytes. J. Natl. Cancer Inst. 49: 891-895, 1972. PubMed: 4567231
22550: Ohsugi Y, et al. Tumorigenicity of human malignant lymphoblasts: comparative study with unmanipulated nude mice, antilymphocyte serum-treated nude mice, and X- irradiated nude mice. J. Natl. Cancer Inst. 65: 715-718, 1980. PubMed: 6932523
22735: Mertelsmann R, et al. T-cell growth factor (interleukin 2) and terminal transferase activity in human leukemias and lymphoblastic cell lines. Blut 43: 99-103, 1981. PubMed: 6942897
23322: Rodrigues NR, et al. p53 mutations in colorectal cancer. Proc. Natl. Acad. Sci. USA 87: 7555-7559, 1990. PubMed: 1699228
23337: Sandstrom PA, Buttke TM. Autocrine production of extracellular catalase prevents apoptosis of the human CEM T-cell line in serum-free medium. Proc. Natl. Acad. Sci. USA 90: 4708-4712, 1993. PubMed: 8506323
