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B16F10 小鼠黑色素瘤高轉(zhuǎn)移細(xì)胞

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產(chǎn)品名稱: B16F10 小鼠黑色素瘤高轉(zhuǎn)移細(xì)胞
產(chǎn)品型號(hào): CRL-6475
產(chǎn)品廠商: 美國標(biāo)準(zhǔn)生物品收藏中心（ATCC）
產(chǎn)品文檔: 無相關(guān)文檔


簡單介紹

CRL-6475 B16F10 小鼠黑色素瘤高轉(zhuǎn)移細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范，提供的細(xì)胞株背景清楚，提供參考文獻(xiàn)和培養(yǎng)條件!

B16F10 小鼠黑色素瘤高轉(zhuǎn)移細(xì)胞 的詳細(xì)介紹
CRL-6475 B16F10 小鼠黑色素瘤高轉(zhuǎn)移細(xì)胞
ATCC® Number: CRL-6475?    Price: $269.00
Designations: B16-F10
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus (mouse)
Morphology: melanocyte
CRL-6475 B16F10 小鼠黑色素瘤高轉(zhuǎn)移細(xì)胞
Source: Organ: skin
Strain: C57BL/6J
Disease: melanoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Applications: transfection host (technology from amaxa)
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

      Subcultivation Ratio: A subcultivation ratio of 1:10 is recommended
      Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
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