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BALB/3T3 clone A31小鼠胚胎成纖維細(xì)胞

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產(chǎn)品名稱: BALB/3T3 clone A31小鼠胚胎成纖維細(xì)胞
產(chǎn)品型號: CCL-163A31小鼠胚胎成纖維細(xì)胞
產(chǎn)品廠商: 美國標(biāo)準(zhǔn)生物品收藏中心（ATCC）
產(chǎn)品文檔: 無相關(guān)文檔


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BALB/3T3 clone A31小鼠胚胎成纖維細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范，提供的細(xì)胞株背景清楚，提供參考文獻(xiàn)和培養(yǎng)條件!BALB/3T3 clone A31小鼠胚胎成纖維細(xì)胞

BALB/3T3 clone A31小鼠胚胎成纖維細(xì)胞 的詳細(xì)介紹
BALB/3T3 clone   A31小鼠胚胎成纖維細(xì)胞
ATCC® Number: CCL-163?    Price: $269.00
Designations: BALB/3T3 clone A31
Depositors:  S Aaronson
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus (mouse)
Morphology: fibroblast
BALB/3T3 clone A31小鼠胚胎成纖維細(xì)胞
Source: Organ: embryo
Strain: BALB/c
Cell Type: fibroblast
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Isolation: Isolation date: 1968
Applications: testing [92346] [92389]
transfection host (Roche FuGENE® Transfection Reagents)
Virus Susceptibility: Herpes simplex virus
Vesicular stomatitis virus
Human poliovirus 1
Tumorigenic: No
Reverse Transcript: negative
Cytogenetic Analysis: modal number = 78; range = 62 to 109.
The stemline number is hypotetraploid. Most of the cells contained only telocentric or acrocentric chromosomes.
Age: embryo; 14 to 17 day gestation
Comments: The BALB/3T3 clone A31 is one of several cell lines (see ATCC CCL-164, BALB/3T12) developed by S.A. Aaronson and G.T. Todaro in 1968 from disaggregated 14- to 17-day-old BALB/c mouse embryos [PubMed: 4301006].The cells are extremely sensitive to contact inhibition of cell division, grow at a high dilution, exhibit a low saturation density and are highly susceptible to transformation in tissue culture by the oncogenic DNA virus SV40 and murine sarcoma virus [PubMed: 4306523].
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: bovine calf serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Growth Conditions: The serum used is calf serum, NOT fetal calf serum. The depositor recommended calf serum because fetal calf serum causes transformation and loss of contact inhibition.
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