產(chǎn)品[
D341 Med 人髓母細(xì)胞瘤
]資料
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產(chǎn)品名稱:
D341 Med 人髓母細(xì)胞瘤
產(chǎn)品型號:
HTB-187
產(chǎn)品廠商:
美國標(biāo)準(zhǔn)生物品收藏中心（ATCC）
產(chǎn)品文檔:
無相關(guān)文檔
簡單介紹
D341 Med 人髓母細(xì)胞瘤，原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種；細(xì)胞庫管理規(guī)范，提供的細(xì)胞株背景清楚，提供參考文獻(xiàn)和Z優(yōu)培養(yǎng)條件！
D341 Med 人髓母細(xì)胞瘤
的詳細(xì)介紹
D341 Med 人髓母細(xì)胞瘤
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HTB-187?
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$323.00
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D341 Med
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HS Friedman
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Biosafety Level:
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1
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frozen
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See Propagation
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suspension
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Homo sapiens (human)
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spheroid
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Organ: brain
Tissue: cerebellum
Disease: medulloblastoma
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neurofilament proteins
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
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Yes
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Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 11,13
D16S539: 12,14
D5S818: 11,12
D7S820: 9,13
F13A01: 6,7
F13B: 8,10
FESFPS: 10,13
LPL: 11
THO1: 6,9.3
TPOX: 8,11
vWA: 17,18
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This is a hyperdiploid (modal number = 49; range = 44 to 50) human cell line with higher ploidies occurring with a frequency of 8.5%. Four marker chromosomes are present in all cells. They are: 11HSR, i(17q), der(22)t(1;22)(q12;p12) and 8q-. There are three copies each of N6 and N18, and single copies each of N11, N17 and N22. The single X chromosome was structurally normal, and the presence of the Y chromosome was confirmed by fluorescence microsopy.
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3.5 years
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male
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ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Temperature: 37.0°C
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Protocol: Cultures can be maintained by addition or replacement of medium. Adherent cells can be dislodged by scraping, and cultures can be established by resuspending a cell pellet at 5 X 10(5) viable cells/ml. Maintain cultures between 4 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: 2 to 3 times per week
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Freeze medium: culture medium, 90%; DMSO, 10%
Storage temperature: liquid nitrogen vapor phase
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