產(chǎn)品[
CCL-257 NCI-H1688 人經(jīng)典小細(xì)胞肺癌細(xì)胞
]資料
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產(chǎn)品名稱:
CCL-257 NCI-H1688 人經(jīng)典小細(xì)胞肺癌細(xì)胞
產(chǎn)品型號:
CCL-257
產(chǎn)品廠商:
美國標(biāo)準(zhǔn)生物品收藏中心（ATCC）
產(chǎn)品文檔:
無相關(guān)文檔
簡單介紹
CCL-257 NCI-H1688 人經(jīng)典小細(xì)胞肺癌細(xì)胞，原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種；細(xì)胞庫管理規(guī)范，提供的細(xì)胞株背景清楚，提供參考文獻(xiàn)和培養(yǎng)條件！
CCL-257 NCI-H1688 人經(jīng)典小細(xì)胞肺癌細(xì)胞
的詳細(xì)介紹
CCL-257 NCI-H1688 人經(jīng)典小細(xì)胞肺癌細(xì)胞
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CCL-257?
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$417.00
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NCI-H1688 [H1688]
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AF Gazdar, JD Minna
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Biosafety Level:
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1
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frozen
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See Propagation
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adherent
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Homo sapiens (human)
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epithelial
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Organ: lung
Disease: carcinoma; classic small cell lung cancer
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
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Amelogenin: X
CSF1PO: 12
D13S317: 8
D16S539: 13
D5S818: 10
D7S820: 12
THO1: 6,8
TPOX: 11
vWA: 17
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50 years
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male
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Caucasian
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ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
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Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Remove spent medium, and add fresh 0.25% trypsin, 0.03% EDTA solution for 2 to 3 minutes at room temperature.
Remove the trypsin and incubate the flask at 37C for 5 to 10 minutes or until the cells detach.
Add fresh medium, aspirate and dispense into new flasks.
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Culture medium, 95%; DMSO, 5%
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Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
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