產(chǎn)品[
HTB-107 SW579 人甲狀腺鱗癌細胞
]資料
如果您對該產(chǎn)品感興趣的話,可以
產(chǎn)品名稱:
HTB-107 SW579 人甲狀腺鱗癌細胞
產(chǎn)品型號:
HTB-107
產(chǎn)品廠商:
美國標準生物品收藏中心（ATCC）
產(chǎn)品文檔:
無相關文檔
簡單介紹
HTB-107 SW579 人甲狀腺鱗癌細胞，原代細胞|細胞系|細胞株|菌種；細胞庫管理規(guī)范，提供的細胞株背景清楚，提供參考文獻和培養(yǎng)條件！
HTB-107 SW579 人甲狀腺鱗癌細胞
的詳細介紹
HTB-107 SW579 人甲狀腺鱗癌細胞
|
|
HTB-107?
|
|
$417.00
|
|
|
SW579 [SW 579, SW-579]
|
|
|
A Leibovitz
|
|
Biosafety Level:
|
1
|
|
|
frozen
|
|
|
See Propagation
|
|
|
adherent
|
|
|
Homo sapiens (human)
|
|
|
epithelial
|
|
|
Organ: thyroid
Disease: squamous cell carcinoma
|
|
|
In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
|
|
|
|
|
Isolation date: 1973
|
|
|
Yes
|
|
|
blood type O; Rh+
|
|
|
Amelogenin: X
CSF1PO: 13
D13S317: 13
D16S539: 11
D5S818: 11
D7S820: 8,9
THO1: 8,9.3
TPOX: 8,10
vWA: 14,18
|
|
|
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 1-2
PGM1, 1-2
PGM3, 1
|
|
|
59 years
|
|
|
male
|
|
|
Caucasian
|
|
|
ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air,
Temperature: 37.0°C
|
|
|
Protocol:
-
Remove and discard culture medium.
-
Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
-
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
-
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
-
Add appropriate aliquots of the cell suspension to new culture vessels.
-
Incubate cultures at 37C.
Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:10 is recommended
Medium Renewal: 2 to 3 times per week
|
|
|
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
|
|
|
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008
recommended serum:ATCC 30-2020
|
|
|
22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080
|
|
