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Designations:
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WERI-Rb-1
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Depositors:
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TW Sery
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Biosafety Level:
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1
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Shipped:
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frozen
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Medium & Serum:
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See Propagation
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Growth Properties:
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suspension
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Organism:
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Homo sapiens
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Morphology:
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grape-like clusters of round cells
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Source:
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Organ: eye
Tissue: retina
Disease: retinoblastoma
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Permits/Forms:
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
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Isolation:
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Isolation date: 1974
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Applications:
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transfection host
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Tumorigenic:
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Yes
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Cytogenetic Analysis:
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This is a near diploid line. The modal chromosome number is 47 occurring at 38%, and the rate of polyploidy is 9%. Fifteen to sixteen marker chromosomes are present in all cells. They are t(1,?), t(3p,5q), der(3)t(?q29;?), t(3q,?), 5q+, i(6p), t(7q,?), 9q+, t(10q,21q), 16q+ and five to six others. Normal chromosomes 3, 10, 13 and 16 are absent. There are two copies of the X chromosome. No Y chromosomes were detected in QM stained preparations
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Isoenzymes:
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ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 1
PGM1, 1
PGM3, 0
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Age:
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1 year
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Gender:
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female
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Ethnicity:
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Caucasian
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Propagation:
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ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: 5% CO2 in air recommended
Temperature: 37.0°C
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Subculturing:
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Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 2 - 3 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp5 and 1 to 2 X 10 exp6 viable cells/ml.
Medium Renewal: Every 3 to 4 days
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Preservation:
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Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
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Related Products:
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Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
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References:
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23026: McFall RC, et al. Characterization of a new continuous cell line derived from a human retinoblastoma. Cancer Res. 37: 1003-1010, 1977. PubMed: 844036
23030: McFall RC, et al. Scanning electron microscopic observation of two retinoblastoma cell lines. Cancer Res. 38: 2827-2835, 1978. PubMed: 679190
32287: Rostomily RC, et al. Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res. 57: 3526-3531, 1997. PubMed: 9270024
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