COLO 320HSR 人結(jié)腸癌細胞
COLO 320DM (ATCC® CCL-220?)
Organism Homo sapiens, human
Tissue colon
Product Format frozen
Morphology rounded and refractile
Culture Properties mixed, adherent and suspension
Biosafety Level 1
Disease Dukes' type C, colorectal adenocarcinoma
Age 55 years
Gender female
Ethnicity Caucasian
Storage Conditions
Karyotype modal number = 24; range = 49 to 61.
The stemline chromosome number is hyperdiploid and 9-11 marker chromosomes (M1, M2, M3, M5, M10, M11, M12, M13, M14, 7p- and 10 q+) were common. Double minutes were observed in 24% of the metaphases examined.
Images
Clinical Data 55 years
Caucasian
female
Genes Expressed serotonin; norepinephrine; epinephrine; adrenocorticotropic hormone (ACTH); parathyroid hormone
Tumorigenic Yes
Effects Yes, in nude mice
Comments The line is negative for CEA, CSAp and colon antigen 3.
The cells are weakly positive for keratins and vimentin
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Shake flask, pour one-half of the medium into a new flask and add fresh medium to both flasks. Cells remaining attached may be removed (if desired) using a standard trypsin protocol.
1.Add 2.0 to 3.0 mL of 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed.
2.Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
3.Add appropriate aliquots of the cell suspension to new culture vessels.
4.Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation Freeze medium: Complete growth medium supplemented with 10% (v/v) DMSO
Storage temperature: Liquid nitrogen vapor phase
Culture Conditions Temperature: 37°C
STR Profile Amelogenin: X
CSF1PO: 11
D13S317: 11
D16S539: 11,12
D5S818: 12
D7S820: 9,12
THO1: 9
TPOX: 8,9
vWA: 15,18
Isoenzymes ES-D, 1
G6PD, B
PEP-D, 1
PGD, A
PGM1, 1
PGM3, 2
Name of Depositor GE Moore
References Clevenstine EC, et al. Cell lines from human colon carcinoma with unusual cell products, double minutes, and homogeneously staining regions. Cancer Res. 39: 4914-4924, 1979. PubMed: 498117
Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874
References Clevenstine EC, et al. Cell lines from human colon carcinoma with unusual cell products, double minutes, and homogeneously staining regions. Cancer Res. 39: 4914-4924, 1979. PubMed: 498117
Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874
